Collagen IV antibody

Catalog No. ABIN335193

Cited in 4+ publications. The Rabbit Polyclonal anti-Collagen IV antibody (ABIN335193) specifically detects Collagen IV in WB, IHC, IHC (fro), IHC (p) and ICC. The antibody is reactive with Human, Rat, Pig and Cow samples.

Quick Overview for Collagen IV antibody (ABIN335193)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human, Rat, Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Collagen IV Antibody

Specificity: Human, rat, pig, cow.

Purification: Antiserum

Immunogen: This polyclonal type IV collagen antibody is a rabbit polyclonal antibody. The rabbits were immunized with isolated human type IV collagen. The immunogen has been isolated from human placenta.

Application Details

Application Notes: Polyclonal type IV collagen recognizes the extracellular - basement membranes. Polyclonal type IV is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl 2. For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration, 1:10 - 1:20 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

References for anti-Collagen IV antibody (ABIN335193)

Cleutjens, Havenith, Kasper, Vallinga, Bosman: "Absence of type IV collagen in the centre of the corneal epithelial basement membrane." in: The Histochemical journal, Vol. 22, Issue 12, pp. 688-94, (1991) (PubMed).

Schapers, Pauwels, Havenith, Smeets, van den Brandt, Bosman: "Prognostic significance of type IV collagen and laminin immunoreactivity in urothelial carcinomas of the bladder." in: Cancer, Vol. 66, Issue 12, pp. 2583-8, (1991) (PubMed).

Cleutjens, Havenith, Beek, Vallinga, Ten Kate, Bosman: "Origin of basement membrane type IV collagen in xenografted human epithelial tumor cell lines." in: The American journal of pathology, Vol. 136, Issue 5, pp. 1165-72, (1990) (PubMed).

Visser, van der Beek, Havenith, Cleutjens, Bosman: "Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia." in: Histopathology, Vol. 10, Issue 2, pp. 171-80, (1986) (PubMed).

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV