Vimentin antibody (VIM)

Details for Product anti-VIM Antibody No. ABIN335381, Supplier: Log in to see
Antigen
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Human, Rat (Rattus), Goat, Dog (Canine), Hamster, Pig (Porcine)
1021
427
386
259
168
159
146
115
107
76
63
50
47
42
28
27
24
14
9
6
3
2
2
2
1
1
1
1
1
1
1
Host
Mouse
618
367
31
14
12
11
11
5
Clonality (Clone)
Monoclonal ()
Conjugate
This Vimentin antibody is un-conjugated
35
31
26
25
24
16
15
12
12
12
12
12
12
12
12
9
9
7
7
7
3
3
3
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3
3
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3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
803
418
369
335
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225
134
86
63
40
26
15
13
8
7
6
3
3
3
3
2
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen RV203 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
Clone RV203
Isotype IgG1
Specificity Human, rat, goat, canine, hamster and swine.
Purification Purified
Plasmids, Primers & others Plasmids, Primers & others Vimentin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Vimentin
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Pathways Caspase Cascade in Apoptosis
Application Notes RV203 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV203 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only
Storage 4 °C
Supplier Images
 image for anti-Vimentin (VIM) antibody (ABIN335381) anti-Vimentin (VIM) antibody
 image for anti-Vimentin (VIM) antibody (ABIN335381) anti-Vimentin (VIM) antibody (Image 2)
Product cited in: Schaart, Pieper, Kuijpers, Bloemendal, Ramaekers: "Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins." in: Differentiation; research in biological diversity, Vol. 46, Issue 2, pp. 105-15, 1991 (PubMed).

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