Vimentin antibody (VIM)

Details for Product anti-VIM Antibody No. ABIN335381
Antigen
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Alternatives
anti-Human Vimentin antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Human, Rat (Rattus), Goat, Dog (Canine), Hamster, Pig (Porcine)
843
324
309
128
117
77
64
57
55
41
39
38
19
15
12
10
9
6
5
3
2
2
2
2
1
1
1
1
1
1
Host
Mouse
410
403
29
12
11
10
10
5
Clonality (Clone)
Monoclonal ()
Conjugate
This Vimentin antibody is un-conjugated
37
24
21
16
14
11
11
11
11
11
11
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
651
333
229
213
194
185
92
63
62
58
41
24
15
13
8
7
3
3
3
2
1
1
1
1
1
Options
Immunogen RV203 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
Clone RV203
Isotype IgG1
Specificity Human, rat, goat, canine, hamster and swine.
Purification Purified
Plasmids, Primers & others Plasmids, Primers & others Vimentin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Pathways Caspase Cascade in Apoptosis
Application Notes RV203 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV203 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only
Storage 4 °C
Supplier Images
 image for anti-Vimentin (VIM) antibody (ABIN335381) anti-Vimentin (VIM) antibody
 image for anti-Vimentin (VIM) antibody (ABIN335381) anti-Vimentin (VIM) antibody (Image 2)
Product cited in: Schaart, Pieper, Kuijpers, Bloemendal, Ramaekers: "Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins." in: Differentiation; research in biological diversity, Vol. 46, Issue 2, pp. 105-15, 1991 (PubMed).

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