7KC-Modified Keyhole-Lympet Hemocyanine antibody

Catalog No. ABIN335416

Product Details

Target: 7KC-Modified Keyhole-Lympet Hemocyanine

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (IHC)

Specificity: Specific for immunohistochemical detection of 7KC in atherosclerotic aorta.

Purification: Protein A purified

Clone: 35A

Isotype: IgG

Application Details

Application Notes: It is recommended that the antibody be tried at 5-10 µg/mL on frozen tissue. Not applicable to paraffin embedded tissues.

Restrictions: For Research Use only

Handling

Format: Frozen

Concentration: 1 mg/mL

Buffer: 10 mM PBS containing 0.1 % NaN3 and 0.1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Moreira, Larrayoz, Lee, Rodríguez: "7-Ketocholesterol is present in lipid deposits in the primate retina: potential implication in the induction of VEGF and CNV formation." in: Investigative ophthalmology & visual science, Vol. 50, Issue 2, pp. 523-32, (2009) (PubMed).

Rodriguez, Fliesler: "Photodamage generates 7-keto- and 7-hydroxycholesterol in the rat retina via a free radical-mediated mechanism." in: Photochemistry and photobiology, Vol. 85, Issue 5, pp. 1116-25, (2009) (PubMed).

Target Details

Target: 7KC-Modified Keyhole-Lympet Hemocyanine

Target Type: Chemical

Background: About 7-ketocholesterol (7-KC) Cholesterol oxidation products, especially 7-Ketocholesterol (7KC), have been the focus of much attention because they are present in human atherosclerotic plaque and display a wide range of atherogenic properties in vitro and to some extant in vivo. This antibody is specific for 7KC. This antibody is useful to detect 7KC in tissue samples. A frozen tissue sample is recommended for immunohistochemistry.