Smooth Muscle Actin antibody

Catalog No. ABIN336852

This anti-Smooth Muscle Actin antibody is a Mouse Monoclonal antibody detecting Smooth Muscle Actin in IHC and IHC (p). Suitable for Human, Rat, Mouse, Chicken, Cow and Baboon.

Quick Overview for Smooth Muscle Actin antibody (ABIN336852)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human, Rat, Mouse, Chicken, Cow, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1A4

Product Details anti-Smooth Muscle Actin Antibody

Specificity: Acetyl group with N- terminal four amino acids of peptide chain of alpha smooth muscle.

Immunogen: N-terminal decapeptide of alpha smooth muscle isoform of actin, acetylated at the N-terminus

Isotype: IgG1

Application Details

Application Notes: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections and cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. The optimum dilution should be determined by the individual lab. Positive Control: Human uterus, gastrointestinal tissue, Leiomyosarcoma. Cellular Localization: Cytoplasmic.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The concentrated antibodies are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: a-Actin alpha smooth muscle

Background: Actin is a globular, roughly 42-kDa protein found in all eukaryotic cells. Actin participates in many important cellular functions, including muscle contraction cell motility cell division and cytokinesis vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Expression of smooth muscle alpha actin is regulated by hormones and cell proliferation and is altered by pathological conditions including onogenic transformation and atherosclerosis.

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development