POLL antibody (AA 563-575)

Catalog No. ABIN337014

The Goat Polyclonal anti-POLL antibody (ABIN337014) specifically detects POLL in ELISA, IHC and IHC (p). The antibody is reactive with Human, Rat, Mouse, Rabbit, Cow, Pig, Bat, Hamster and Monkey samples.

Quick Overview for POLL antibody (AA 563-575) (ABIN337014)

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Reactivity: Human, Rat, Mouse, Rabbit, Cow, Pig, Bat, Hamster, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This POLL antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POLL Antibody

Binding Specificity: AA 563-575

Specificity: Human POLL. It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32 % in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Pig (100%) Dog, Opossum, Turkey, Chicken (92%) Xenopus, Zebrafish, Sea anemone (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-LGLPYREPAERDW from the C-terminus of human POLL (NP_037406.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Pig (100%), Dog, Opossum, Turkey, Chicken (92%), Xenopus, Zebrafish, Sea anemone (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for POLL

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Alternative Name: POLL / DNA Polymerase Lambda

Background: Name/Gene ID: POLL

Synonyms: POLL, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase lambda, DNA polymerase kappa, Pol beta2, BETAN, Pol Lambda, POLKAPPA

Gene ID: 27343

NCBI Accession: NP_037406

UniProt: Q9UGP5

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response