NCF1 antibody (AA 378-390)

Catalog No. ABIN337093

This Goat Polyclonal antibody specifically detects NCF1 in WB, IHC, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Monkey and Cow.

Quick Overview for NCF1 antibody (AA 378-390) (ABIN337093)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human, Monkey, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NCF1 Antibody

Binding Specificity: AA 378-390

Specificity: Human NCF1 / p47phox.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey, Marmoset, Dolphin, Elephant, Panda, Bovine (100%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SESTKRKLASAV from the C-terminus of human NCF1 / p47phox (NP_000256.3). Percent identity by BLAST analysis: Human, Monkey, Marmoset, Dolphin, Elephant, Panda, Bovine (100%), Mouse, Rat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IF, IHC, IHC-P (5 μg/mL), Peptide-ELISA, WB (0.2 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: NCF1 / p47phox / p47 Phox

Background: Name/Gene ID: NCF1

Synonyms: NCF1, NCF1A, Neutrophil cytosolic factor 1, NCF-47K, Nox organizer 2, NOXO2, p47-phox, p47phox, SH3PXD1A, NADPH oxidase organizer 2, NCF-1, Neutrophil cytosol factor 1, Nox-organizing protein 2

Gene ID: 653361

NCBI Accession: NP_000256

UniProt: P14598

Pathways: PI3K-Akt Signaling