beta-2 Microglobulin antibody

Catalog No. ABIN337153

This Mouse Monoclonal antibody specifically detects beta-2 Microglobulin in WB, IHC, FACS, ELISA, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for beta-2 Microglobulin antibody (ABIN337153)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-beta-2 Microglobulin Antibody

Specificity: Recognizes human b2-microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as soluble human b2-microglobulin. Does not cross-react with mouse, bovine, rabbit, dog or chicken b2-microglobulin.

Purification: Ion exchange chromatography

Immunogen: Purified human b2-microglobulin.

Type of Immunogen: Purified protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, Flo (1 μg/mL), IHC, IHC-P (10 μg/mL), IP, WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: B2M / Beta 2 Microglobulin

Background: Name/Gene ID: B2M

Synonyms: B2M, Beta 2 Microglobulin, Beta-2-microglobulin, Beta-2-microglobin

Gene ID: 567

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process