JAG1 antibody (AA 110-125)

Catalog No. ABIN337168

This Rabbit Polyclonal antibody specifically detects JAG1 in WB, IHC, ELISA, ICC, IF and IHC (p). It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for JAG1 antibody (AA 110-125) (ABIN337168)

Target: JAG1 (Jagged 1 (JAG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-JAG1 Antibody

Binding Specificity: AA 110-125

Specificity: Recognizes human Jagged1 (JAG1).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to aa 110-125 of human Jagged-1protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:2500 - 1:10000), ICC, IF (1:100 - 1:500), IHC, IHC-P (5 μg/mL), WB (1:250 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

References for anti-JAG1 antibody (ABIN337168)

Siar, Ha, Aung, Nakano, Tsujigiwa, Nagatsuka, Ng, Kawakami: "Immunolocalization of notch signaling protein molecules in a maxillary chondrosarcoma and its recurrent tumor." in: European journal of medical research, Vol. 15, Issue 10, pp. 456-60, (2010) (PubMed).

Target Details for JAG1

Target: JAG1 (Jagged 1 (JAG1))

Alternative Name: JAG1 / Jagged 1

Background: Name/Gene ID: JAG1

Synonyms: JAG1, AGS, AHD, Alagille syndrome, CD339 antigen, HJ1, Jagged1, JAGL1, Jagged, Jagged 1, Protein jagged-1, AWS, CD339, Soluble protein jagged

Gene ID: 182

UniProt: P78504

Pathways: Notch Signaling, Stem Cell Maintenance