Tyrosine Hydroxylase antibody (pSer40)

Catalog No. ABIN337188

The Rabbit Polyclonal anti-Tyrosine Hydroxylase antibody (ABIN337188) specifically detects Tyrosine Hydroxylase in WB, IHC, IF, IHC (p) and IHC (fro). The antibody is reactive with Human and Rat samples.

Quick Overview for Tyrosine Hydroxylase antibody (pSer40) (ABIN337188)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Tyrosine Hydroxylase Antibody

Binding Specificity: pSer40

Specificity: Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.

Purification: Immunoaffinity purified

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer40 of rat tyrosine hydroxylase.

Application Details

Application Notes: Approved: IF (1:1000), IHC, IHC-Fr (1:1000), IHC-P (5 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Storage: -20 °C,-80 °C

Storage Comment: Short term: -20°C
Long term: -80°C.

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: TH / Tyrosine Hydroxylase

Background: Name/Gene ID: TH
Family: Monooxygenase

Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase

Gene ID: 7054

UniProt: P07101

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour