Insulin antibody

Catalog No. ABIN337249

This Rabbit Polyclonal antibody specifically detects Insulin in IHC, IF, IHC (p), FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Insulin antibody (ABIN337249)

Target: Insulin (INS)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Insulin Antibody

Brand: IHC-plus™

Specificity: Recognizes endogenous levels of total human insulin protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (KLH-coupled) derived from the sequence of human insulin.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: Flo, ICC, IF, IHC, IHC-P (1:100)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Name/Gene ID: INS
Family: Hormone

Synonyms: INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin

Gene ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation