OGT antibody (AA 543-555)

Catalog No. ABIN337257

The Goat Polyclonal anti-OGT antibody (ABIN337257) specifically detects OGT in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Bat, Monkey, Pig and Hamster samples.

Quick Overview for OGT antibody (AA 543-555) (ABIN337257)

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Bat, Monkey, Pig, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This OGT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-OGT Antibody

Binding Specificity: AA 543-555

Specificity: Human OGT. This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%) Xenopus (92%) Turkey, Chicken, Pufferfish, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-YEHPKDLKLSDGR from an internal region of human OGT (NP_858058.1, NP_858059.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%), Xenopus (92%), Turkey, Chicken, Pufferfish, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (1:64000, 0.05 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for OGT

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Alternative Name: OGT / O-GLCNAC

Background: Name/Gene ID: OGT

Synonyms: OGT, HRNT1, O-GLCNAC

Gene ID: 8473

NCBI Accession: NP_858058, NP_858059

UniProt: O15294

Pathways: Regulation of Carbohydrate Metabolic Process