Angiotensin I Converting Enzyme 1 antibody

Catalog No. ABIN337280

The Mouse Monoclonal anti-Angiotensin I Converting Enzyme 1 antibody is suitable to detect Angiotensin I Converting Enzyme 1 in samples from Human, Mouse, Rat, Dog, Cow, Rabbit, Monkey, Cat and Guinea Pig. It has been validated for WB, IHC and IHC (p).

Quick Overview for Angiotensin I Converting Enzyme 1 antibody (ABIN337280)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Monkey, Cat, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Angiotensin I Converting Enzyme 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Angiotensin I Converting Enzyme 1 Antibody

Specificity: Denatured Angiotensin-converting enzyme (ACE). Species cross-reactivity: Human, monkey, rabbit, bovine, canine, feline, guinea pig, rat and mouse.

Purification: Purified

Immunogen: Angiotensin-converting enzyme (denatured) from human kidney.

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid. No preservatives added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Angiotensin I Converting Enzyme 1

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Alternative Name: ACE / DCP1 / CD143

Background: Name/Gene ID: ACE
Subfamily: Metallopeptidase M2
Family: Protease

Synonyms: ACE, ACE1, Angiotensin-converting enzyme, CD143, CD143 antigen, Carboxycathepsin, DIPEPTIDYL CARBOXYPEPTIDASE, DCP, Dipeptidyl carboxypeptidase I, ICH, Kininase II, MVCD3, Peptidyl-dipeptidase A, Peptidase P, DCP1, Dipeptidyl carboxypeptidase 1, Testicular ECA

Gene ID: 1636

UniProt: P12821

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration