CAMK2B antibody (pThr286)

Catalog No. ABIN337356

The Rabbit Polyclonal anti-CAMK2B antibody (ABIN337356) specifically detects CAMK2B in WB, IHC and IHC (p). The antibody is reactive with Human, Rat, Mouse and Xenopus laevis samples.

Quick Overview for CAMK2B antibody (pThr286) (ABIN337356)

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Reactivity: Human, Rat, Mouse, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMK2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CAMK2B Antibody

Binding Specificity: pThr286

Specificity: Specific for the ~50k a-CaM Kinase II and the ~60k R-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the X-phosphatase treatment

Purification: Immunoaffinity purified

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr286 found in rat brain CaM Kinase II.

Application Details

Application Notes: Approved: IHC, IHC-P (1:200), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:200.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA, 50 % glycerol

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CAMK2B

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Alternative Name: CAMK2 / CAMKII

Background: Name/Gene ID: CAMK2

Synonyms: CAMK2

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity