Collagen Type I antibody

Catalog No. ABIN344731

This Mouse Monoclonal antibody specifically detects Collagen Type I in ELISA, IHC, IHC (fro) and RIA. It exhibits reactivity toward Human.

Quick Overview for Collagen Type I antibody (ABIN344731)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Product Details anti-Collagen Type I Antibody

Specificity: Recognizes both native and heat denatured human collagen type I. No cross-reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5 % binding to collagens II and III in ELISA, RIA or immunoblot.

Purification: Ammonium sulfate precipitation

Immunogen: Human Collagen Type I.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (10 - 20 μg/mL), IHC, IHC-Fr, RIA

Usage: Suitable for use in ELISA, Immunohistochemistry and RIA. Not suitable for Western Blot. ELISA: Use as capture or as a second detecting antibody in sandwich format (Apparent binding constant is ~0.1nM). Immunohistochemistry (acetone or ethanol fixed frozen 10um sections): 10-20 μg/mL, Staining is similar to that for polyclonal antibodies to interstitial collagen I. Not suitable for paraffin-embedded sections or formaldehyde fixation.

Not recommended for: IHC-P, WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, 1 mL sterile distilled water or sterile 40-50 % glycerol.

Concentration: Lot specific

Buffer: Lyophilized powder. Reconstitute, 1 mL sterile distilled water or sterile 40-50 % glycerol.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen I