Prosaposin antibody

Catalog No. ABIN347759

The Rabbit Polyclonal anti-Prosaposin antibody is suitable to detect Prosaposin in samples from Human. It has been validated for WB, ELISA and ICC.

Quick Overview for Prosaposin antibody (ABIN347759)

Target: Prosaposin (PSAP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prosaposin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-Prosaposin Antibody

Specificity: Reacts with human Saposin-B

Cross-Reactivity (Details): May cross react with proteins from other species due to sequence homology

Purification: Antiserum

Immunogen: Synthetic peptide derived from an internal domain of human PSAP

Isotype: IgG

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ICC1:200 - 1:1 000 WB1:500 - 1:5 000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for Prosaposin

Target: Prosaposin (PSAP)

Alternative Name: Proactivator Polypeptide Domain Saposin-B (PSAP)

Background: The lysosomal degradation of sphingolipids takes place by the sequential action of specific hydrolases. Some of these enzymes require specific low-molecular mass, non-enzymic proteins: the sphingolipids activator proteins (coproteins). Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase and galactosylceramide by beta-galactosylceramidase. Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate. Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A, GM1 gangliosides by beta-galactosidase and globotriaosylceramide by alpha-galactosidase A. Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases. Saposin-D is a specific sphingomyelin phosphodiesterase activator.

Gene ID: 5660

UniProt: P07602

Pathways: Positive Regulation of Endopeptidase Activity