GFP antibody
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- Target See all GFP Antibodies
- GFP (Green Fluorescent Protein (GFP))
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Reactivity
- Aequorea victoria
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GFP antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Purpose
- Rabbit antibody to EGFP/GFP/Venus
- Specificity
- Specific for EGFP/GFP/Venus
- Cross-Reactivity (Details)
- Any organism expressing EGFP/GFP/Venus.
- Purification
- IgG
- Immunogen
- Recombinant EGFP/GFP/Venus protein was used as the antigen.
- Isotype
- IgG
- Top Product
- Discover our top product GFP Primary Antibody
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anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, FM Host: Goat Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea aequorea WB, IF, IHC (fro), IHC (p) Host: Goat Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq Host: Rat Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, IHC, FACS Host: Mouse Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM Host: Goat Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, IHC, IP, FACS, FM, DB Host: Mouse Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP Reactivity: Aequorea victoria WB, IF, IHC (fro) Host: Goat Polyclonal DyLight 550
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, IHC, IP, FACS, FM Host: Rabbit Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibody (FITC)GFP Reactivity: Aequorea victoria WB, IHC, FM, DB, FLISA Host: Goat Polyclonal FITC
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- Application Notes
- IHC WB. A working concentration of 5-50 μg,ml is recommended. The optimal concentration should be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
- Handling Advice
- Avoid freeze and thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term.
- Expiry Date
- 12 months
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- Target
- GFP (Green Fluorescent Protein (GFP))
- Abstract
- GFP Products
- Background
- GFP is a naturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin's blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer reporter of gene expression or as a measure of protein-protein interactions.
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