NEDD8 antibody (N-Term)

Catalog No. ABIN350514

This anti-NEDD8 antibody is a Sheep Polyclonal antibody detecting NEDD8 in WB, IHC and IHC (p). Suitable for Mouse.

Quick Overview for NEDD8 antibody (N-Term) (ABIN350514)

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: This NEDD8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NEDD8 Antibody

Binding Specificity: N-Term

Purpose: Sheep antibody to NEDD8

Specificity: Specific for NEDD8.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the n-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8 Neddylin) conjugated to immunogenic carrier protein has been used as the immunogen. The antigen is homologous in human rat zebrafish drosophila and xenopus.

Isotype: IgG

Application Details

Application Notes: IHC (both on frozen,paraffin embedded tissues) WB. Use at a concentration of 10-50,micro,g,ml. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Alternative Name: NEDD8

Background: Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory protein. Subcellular location: Mainly nuclear Tissue specificity: Ubiquitously expressed (at protein level). Post-translational modification: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function

UniProt: P29595

Pathways: Ubiquitin Proteasome Pathway