GRIA2 antibody (AA 175-430)

Catalog No. ABIN351533

The Mouse Monoclonal anti-GRIA2 antibody has been validated for WB, IHC, ELISA and IHC (p). It is suitable to detect GRIA2 in samples from Human, Rat, Mouse and Monkey.

Quick Overview for GRIA2 antibody (AA 175-430) (ABIN351533)

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Reactivity: Human, Rat, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRIA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRIA2 Antibody

Binding Specificity: AA 175-430

Brand: IHC-plus™

Specificity: Recognizes human GluR2 protein (~96kD). No cross-reactivity with other AMPA/Kainate GluR subunits. Species cross-reactivity: mouse, rat and monkey.

Purification: Protein A purified

Immunogen: Fusion protein containing aa175-430 from the N-terminal region of GluR2.

Type of Immunogen: Fusion protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 5 μg/mL), IHC, IHC-P (1:50), WB (1 - 5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GRIA2

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Alternative Name: GRIA2 / GLUR2

Background: Name/Gene ID: GRIA2
Subfamily: Glutamate receptor - ionotropic (NMDA receptor)
Family: Ion Channel

Synonyms: GRIA2, AMPA 2, GluR-K2, Glutamate receptor 2, HBGR2, GluA2, GluR-2, GluR-B, GLUR2, GLURB

Gene ID: 2891

UniProt: P42262

Pathways: PI3K-Akt Signaling