PPARG antibody (AA 1-16)

Catalog No. ABIN351540

This anti-PPARG antibody is a Rabbit Polyclonal antibody detecting PPARG in WB, IHC, ICC, IHC (p) and GS. Suitable for Human and Monkey.

Quick Overview for PPARG antibody (AA 1-16) (ABIN351540)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)

Product Details anti-PPARG Antibody

Binding Specificity: AA 1-16

Brand: IHC-plus™

Specificity: Recognizes mouse PPARg2. Does not cross-react with PPARa or PPARd.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%) Gorilla, Cat, Bat, Dog (94%) Pig (88%) Elephant (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa1-16 (MGETLGDSPIDPESDS) of human PPARg2 (peroxisome proliferator activated receptor g2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%), Gorilla, Cat, Bat, Dog (94%), Pig (88%), Elephant (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: GS, ICC (1:500), IHC, IHC-P (2.5 - 5 μg/mL), WB (1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL. Western Blot: 1:500. Detects a band of ~56kD.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPARG / PPAR Gamma

Background: Name/Gene ID: PPARG
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: PPARG, NR1C3, PPAR gamma, PPAR gamma 2, PPARG1, PPARG2, PPARgamma, CIMT1, GLM1, PPAR-gamma

Gene ID: 5468

UniProt: P37231

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation