Rabbit anti-Human Ig (Chain kappa), (Light Chain) Antibody
IF, IHC (fro), IHC (p)
Polyclonal
IgG
unconjugated
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- Target See all Ig products
- Ig
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Binding Specificity
- Chain kappa, Light Chain
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Reactivity
- Human, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Application
- Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Purified
- Immunogen
- Purified human Kappa light chain from human Bence Jones proteins.
- Isotype
- IgG
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Rabbit anti-Human Ig (Chain kappa), (Light Chain), (N-Term) antibody
IHC (fro), IHC (p) Monoclonal A21-Y unconjugated
Rabbit anti-Human Ig (C-Term), (Chain kappa), (Light Chain) antibodyIHC (fro), IHC (p) Monoclonal H16-E unconjugated
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- Application Notes
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Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate pH 6.0 for10-20 minutes, followed by cooling at RT for 15-20 minutes). Recommended positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 2.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative and 1 % BSA as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- Ig
- Alternative Name
- Immunoglobulin (Ig Products)
- Background
- Human Kappa chain is a 25 kDa light chain present on all 5 immunoglobulin's (IgA,IgG, IgM, IgD, and IgE) and is also present in free form. Kappa and Lambda light chains consists of a variable region and a constant region and can be easily be differentiated by the antigenic properties of a constant region. The ratio of Kappa to Lambda chain is 70:30, most of them are bound to heavy chains of immunoglobulin.
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