PUMA antibody (BH3 Domain, C-Term)

Catalog No. ABIN356830

This Rabbit Polyclonal antibody specifically detects PUMA in WB, IF, FACS, IHC (p) and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PUMA antibody (BH3 Domain, C-Term) (ABIN356830)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PUMA Antibody

Binding Specificity: BH3 Domain, C-Term

Specificity: This antibody is specific to Human and Mouse PUMA (BH3 Domain). Other species not tested.

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: Synthetic peptide selected from the C-terminal region of Human PUMA

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA

Background: PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis

Molecular Weight: 20532 Da

Gene ID: 27113, 9606

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity