CDCA8 antibody (Center)

Catalog No. ABIN356941

This Rabbit Polyclonal antibody specifically detects CDCA8 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for CDCA8 antibody (Center) (ABIN356941)

Target: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDCA8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-CDCA8 Antibody

Binding Specificity: Center

Specificity: This antibody detects CDCA8 at center.

Purification: Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human CDCA8.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blotting 1: 100 - 1: 500. Immunohistochemistry 1: 10 - 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for CDCA8

Target: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Alternative Name: CDCA8 / Borealin

Background: CDCA8 is a component of the chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The CPC complex, a key regulator of mitosis, has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 may be required to direct the CPC to centromeric DNA.Synonyms: Cell division cycle-associated protein 8, Dasra-B, PESCRG3, Pluripotent embryonic stem cell-related gene 3 protein, hDasra-B

Molecular Weight: 31323 Da

Gene ID: 55143, 9606

UniProt: Q53HL2

Pathways: Cell Division Cycle, M Phase