CDCA8 antibody (N-Term)

Catalog No. ABIN499603

This Rabbit Polyclonal antibody specifically detects CDCA8 in WB, IF and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CDCA8 antibody (N-Term) (ABIN499603)

Target: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDCA8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-CDCA8 Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to CDCA8.

Purification: Affinity chromatography purified via peptide column

Immunogen: CDCA8 antibody was raised against a 19 amino acid peptide near the amino terminus of the human CDCA8.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: CDCA8 antibody can be used for detection of CDCA8 at 1 μg/mL. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CDCA8

Target: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Alternative Name: CDCA8 / Borealin

Background: CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.Synonyms: Cell division cycle-associated protein 8, Dasra-B, PESCRG3, Pluripotent embryonic stem cell-related gene 3 protein, hDasra-B

Gene ID: 55143

NCBI Accession: NP_060571

UniProt: Q53HL2

Pathways: Cell Division Cycle, M Phase