Vimentin antibody (Middle Region)
Quick Overview for Vimentin antibody (Middle Region) (ABIN357888)
Target
See all Vimentin (VIM) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- Middle Region
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Specificity
- This antibody detects Vimentin (VIM) (Center).
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Purification
- Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Immunogen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human Vimentin.
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Isotype
- Ig Fraction
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Application Notes
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ELISA: 1/1,000. Western Blot: 1/50-1/100Immunohistochemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Vimentin (VIM)
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Alternative Name
- Vimentin
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Background
- Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Synonyms: VIM
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Molecular Weight
- 53652 Da
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Gene ID
- 7431, 9606
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UniProt
- P08670
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Pathways
- Caspase Cascade in Apoptosis
Target
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