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Vimentin antibody (Middle Region)

The Rabbit Polyclonal anti-Vimentin antibody (ABIN357888) specifically detects Vimentin in WB, IHC (p) and EIA. The antibody is reactive with Human samples.
Catalog No. ABIN357888
$768.00
Plus shipping costs $50.00
0.4 mL
Shipping to: United States
Delivery in 1 to 2 Business Days

Quick Overview for Vimentin antibody (Middle Region) (ABIN357888)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

  • 417
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  • 72
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Human

Host

  • 217
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  • 10
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Rabbit

Clonality

  • 250
  • 183
Polyclonal

Conjugate

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This Vimentin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 39
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    Middle Region

    Specificity

    This antibody detects Vimentin (VIM) (Center).

    Purification

    Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human Vimentin.

    Isotype

    Ig Fraction
  • Application Notes

    ELISA: 1/1,000. Western Blot: 1/50-1/100Immunohistochemistry: 1/10-1/50.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Synonyms: VIM

    Molecular Weight

    53652 Da

    Gene ID

    7431, 9606

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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