PRKDC antibody (pThr2609)

Catalog No. ABIN358415

The Rabbit Polyclonal anti-PRKDC antibody is suitable to detect PRKDC in samples from Human. It has been validated for EIA.

Quick Overview for PRKDC antibody (pThr2609) (ABIN358415)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-PRKDC Antibody

Binding Specificity: pThr2609

Specificity: This antibody detects DNA pThr2609.

Purification: Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T2609 of human DNA- PK.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: DNA-PKcs / PRKDC / XRCC7

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Stem cell surface receptors and activate yeast MAPK pathway.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460

Molecular Weight: 469079 Da

Gene ID: 5591, 9606

UniProt: P78527

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response