APH1A antibody (N-Term)

Catalog No. ABIN358830

The Rabbit Polyclonal anti-APH1A antibody (ABIN358830) specifically detects APH1A in WB, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for APH1A antibody (N-Term) (ABIN358830)

Target: APH1A (Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APH1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-APH1A Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to APH1.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human APH1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for APH1A

Target: APH1A (Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A))

Alternative Name: APH1A

Background: APH1 is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). The gamma-secretase complex is minimally composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, although other components may exist. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.Synonyms: APH-1A, Aph-1 alpha, CGI-78, Gamma-secretase subunit APH-1A, PSF, Presenilin-stabilization factor

Gene ID: 51107, 9606

UniProt: Q96BI3

Pathways: Notch Signaling, Neurotrophin Signaling Pathway