WEE1 antibody (C-Term)

Catalog No. ABIN360579

This anti-WEE1 antibody is a Rabbit Polyclonal antibody detecting WEE1 in WB, IHC (p) and EIA. Suitable for Human.

Quick Overview for WEE1 antibody (C-Term) (ABIN360579)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-WEE1 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to WEE1.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human WEE1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: WEE1 / Wee1-Like Protein Kinase

Background: WEE1 is a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.Synonyms: WEE1hu, Wee1A kinase

Gene ID: 7465, 9606

UniProt: P30291

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase