Periostin antibody

Catalog No. ABIN361362

Product Details anti-Periostin Antibody

Target: Periostin (POSTN)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Periostin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: The antibody has been directly tested for reactivity in chicken, human, mouse and rat. Based on the homology of the immunogen with other species, we expect that this antibody will recognize periostin in most, if not all, species of mammals, birds, amphibians, and fishes. Specific for three periostin bands around ~93 kDa in mouse lung extract. The immunogenic peptide is present in all known splice variants of periostin and therefore this antibody recognizes all known molecular forms of periostin. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigenretrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20).

Cross-Reactivity: Chicken, Human, Mouse (Murine)

Predicted Reactivity: most mammalian, avian, amphibian and fishes

Purification: Antigen Affinity Purified

Immunogen: Synthetic peptide corresponding to amino acid residues from the fasciclin domain 1 of mouse periostin

Application Details

Application Notes: Recommended Dilution: WB: 1:1,000 IHC: 1:100 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: ~ 0.5 mg/ml

Buffer: 100 μL in PBS

Storage: -20 °C

Target Details for Periostin

Target: Periostin (POSTN)

Alternative Name: POSTN (POSTN Products)

Synonyms: fa99h07 antibody, pn antibody, postn antibody, wu:fa99h07 antibody, wu:fc70f09 antibody, zgc:153873 antibody, LOC100008945 antibody, osf-2 antibody, pdlpostn antibody, periostin antibody, OSF-2 antibody, OSF2 antibody, PDLPOSTN antibody, PN antibody, RP11-412K4.1 antibody, A630052E07Rik antibody, AI747096 antibody, Osf2 antibody, PLF antibody, peri antibody, Plf antibody, periostin antibody, periostin, osteoblast specific factor b antibody, periostin, osteoblast specific factor antibody, POSTN antibody, postnb antibody, postn antibody, Postn antibody

Background: Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has recently been shown to promote collagen fibrogenesis, inhibit differentiation of progenitor cells into cardiomyocytes and to be essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008).

Molecular Weight: '93 kDa

Gene ID: 50706

UniProt: Q62009