Vimentin antibody

Catalog No. ABIN361371

Product Details anti-Vimentin Antibody

Target: Vimentin (VIM)

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: Specific for the ~50kDa Vimentin protein. It is expected that it will work on other mammalian tissues.

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Purification: Total IgY fraction

Immunogen: Recombinant human vimentin purified from E. coli

Application Details

Application Notes: Recommended Dilution: WB: 1:1,000 IF: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-Vimentin antibody (ABIN361371)

Kirane, Toombs, Larsen, Ostapoff, Meshaw, Zaknoen, Brekken, Burrows: "Epithelial-mesenchymal transition increases tumor sensitivity to COX-2 inhibition by apricoxib." in: Carcinogenesis, Vol. 33, Issue 9, pp. 1639-46, (2012) (PubMed).

Kirane, Toombs, Ostapoff, Carbon, Zaknoen, Braunfeld, Schwarz, Burrows, Brekken: "Apricoxib, a novel inhibitor of COX-2, markedly improves standard therapy response in molecularly defined models of pancreatic cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 18, Issue 18, pp. 5031-42, (2012) (PubMed).

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: VIM (VIM Products)

Synonyms: CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

Background: Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).

Molecular Weight: '50 kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis