GRIN2B antibody (C-Term)

Catalog No. ABIN361395

This anti-GRIN2B antibody is a Rabbit Polyclonal antibody detecting GRIN2B in WB, IHC, ELISA and IP. Suitable for Rat. This Primary Antibody has been cited in 3+ publications.

Quick Overview for GRIN2B antibody (C-Term) (ABIN361395)

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-GRIN2B Antibody

Binding Specificity: C-Term

Specificity: Specific for endogenous levels of the ~180 kDa NR2B subunit of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Our NMDA NR2B Subunit rabbit polyclonal primary antibody is produced in-house. It detects NMDA NR2B Subunit and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, IP.

Purification: Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Immunogen: Fusion protein from the C-terminal region of the NR2B subunit of the rat NMDA receptor.

Isotype: IgG

Application Details

Application Notes:

Dilution Range: IP: 3 μL per 200 μg lysate

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Reconstitute in 50 μl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Expiry Date: 12 months

References for anti-GRIN2B antibody (ABIN361395)

Brady, Diaz, Iuso, Everett, Valenzuela, Caldwell: "Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 3, pp. 1062-7, (2013) (PubMed).

Hicklin, Wu, Radcliffe, Freund, Goebel-Goody, Correa, Proctor, Lombroso, Browning: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, (2011) (PubMed).

Kurup, Zhang, Xu, Venkitaramani, Haroutunian, Greengard, Nairn, Lombroso: "Abeta-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 17, pp. 5948-57, (2010) (PubMed).

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: GRIN2B

Background:

Synonyms: AW490526 antibody, EIEE27 antibody, Glutamate [NMDA] receptor subunit epsilon 2 antibody, Glutamate [NMDA] receptor subunit epsilon-2 antibody, Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody, Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody, Glutamate receptor ionotropic NMDA2B antibody, Glutamate receptor subunit epsilon 2 antibody, Glutamate receptor ionotropic NMDA2B (epsilon 2) antibody, GRIN 2B antibody, GRIN2B antibody, hNR 3 antibody, hNR3 antibody, MGC142178 antibody, MGC142180 antibody, MRD6 antibody, N methyl D asparate receptor channel subunit epsilon 2 antibody, N methyl D aspartate receptor subtype 2B antibody, N methyl D aspartate receptor subunit 2B antibody, N methyl D aspartate receptor subunit 3 antibody, N-methyl D-aspartate receptor subtype 2B antibody, N-methyl-D-aspartate receptor subunit 3 antibody, NMDA NR2B antibody, NMDA R2B antibody, NMDAR2B antibody, NMDE2 antibody, NMDE2_HUMAN antibody, NME2 antibody, NR2B antibody, NR3 antibody

Description Logic: Our NMDA NR2B Subunit rabbit polyclonal primary antibody is produced . It detects NMDA NR2B Subunit and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, IP.

Gene Name Alternatives: GRIN2B

Molecular Weight: 180 kDa

Gene ID: 24410

UniProt: Q00960

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity