GRIN1/NMDAR1 antibody

Catalog No. ABIN361400

Product Details anti-GRIN1/NMDAR1 Antibody

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN1/NMDAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for the ~120k NR1 subunit, N1 splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Predicted Reactivity: canine, chicken, human, non-human primate, Xenopus

Purification: Antigen Affinity Purified

Immunogen: Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, N1 splice variant insert conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 to 1:2000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized

Storage: -20 °C

Target Details for GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Alternative Name: GRIN1 (GRIN1 Products)

Synonyms: GluN1 antibody, MRD8 antibody, NMDA1 antibody, NMDAR1 antibody, NR1 antibody, nmda1 antibody, nmdar1 antibody, GB19253 antibody, GRIN1 antibody, NMD-R1 antibody, Z16 antibody, GluRdelta1 antibody, GluRzeta1 antibody, M100174 antibody, Nmdar antibody, Rgsc174 antibody, NMDAR1.1 antibody, zgc:152678 antibody, glutamate ionotropic receptor NMDA type subunit 1 antibody, glutamate receptor, ionotropic, N-methyl D-aspartate 1 S homeolog antibody, NMDA receptor 1 antibody, G protein-regulated inducer of neurite outgrowth 1 antibody, glutamate receptor, ionotropic, NMDA1 (zeta 1) antibody, glutamate receptor, ionotropic, N-methyl D-aspartate 1a antibody, GRIN1 antibody, grin1.S antibody, grin1 antibody, Grin1 antibody, Nmdar1 antibody, Gprin1 antibody, grin1a antibody

Background: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994, Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR. Anti-NMDAR, NR1 Subunit, N1 Splice Variant Insert Western blot of 10 ug of HEK 293 cells expressing: Lane 1 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the N1 splice variant insert., Lane 2 - NR1 subunit containing only the C2 Insert, Lane 3 - NR1 subunit containing the C1 and C2' Insert, Lane 4 - without NR1 expression (Mock).

Molecular Weight: '120 kDa

Gene ID: 24408

UniProt: P35439

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity