Tyrosine Hydroxylase antibody

Catalog No. ABIN361413

Product Details anti-Tyrosine Hydroxylase Antibody

Target: Tyrosine Hydroxylase (TH)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: Specific for the ~60k tyrosine hydroxylase protein.

Cross-Reactivity: Mammalian

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IF (frozen sections, Xu et al., 1998): 1:1000 IHC (frozen sections, Xu et al., 1998): 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-Tyrosine Hydroxylase antibody (ABIN361413)

Sanyal, Dutta, Camara, Chandran, Koller, Watson, Sengupta, Ysselstein, Montenegro, Cannon, Rochet, Mattoo: "Alpha-Synuclein Is a Target of Fic-Mediated Adenylylation/AMPylation: Possible Implications for Parkinson's Disease." in: Journal of molecular biology, Vol. 431, Issue 12, pp. 2266-2282, (2020) (PubMed).

Brindley, Bauer, Walker, Quinlan, Carneiro, Sze, Blakely, Currie: "Adrenal serotonin derives from accumulation by the antidepressant-sensitive serotonin transporter." in: Pharmacological research, Vol. 140, pp. 56-66, (2019) (PubMed).

de Rus Jacquet, Timmers, Ma, Thieme, McCabe, Vest, Lila, Rochet: "Lumbee traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson's disease-related symptoms." in: Journal of ethnopharmacology, Vol. 206, pp. 408-425, (2018) (PubMed).

de Rus Jacquet, Tambe, Ma, McCabe, Vest, Rochet: "Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson's disease-related symptoms." in: Journal of ethnopharmacology, Vol. 206, pp. 393-407, (2018) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine Hydroxylase (TH Products)

Synonyms: DYT14 antibody, DYT5b antibody, TYH antibody, The antibody, CG10118 antibody, DH65B antibody, DTH antibody, Dmel\\CG10118 antibody, Pale antibody, Ple antibody, TH antibody, Th antibody, VII antibody, dTH1 antibody, dTH65B antibody, pale/ple antibody, th antibody, dyt14 antibody, dyt5b antibody, tyh antibody, tyrosine hydroxylase antibody, pale antibody, tyrosine 3-monooxygenase antibody, tyrosine hydroxylase S homeolog antibody, TH antibody, Th antibody, ple antibody, th antibody, th.S antibody, LOC100008895 antibody

Background: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001, Dunkley et al., 2004). Anti-Tyrosine Hydroxylase Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k TH protein.

Molecular Weight: '60 kDa

Gene ID: 25085

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour