GRIN2B antibody (pTyr1472)

Catalog No. ABIN361485

This anti-GRIN2B antibody is a Rabbit Polyclonal antibody detecting GRIN2B in WB and ELISA. Suitable for Rat. This Primary Antibody has been cited in 6+ publications.

Quick Overview for GRIN2B antibody (pTyr1472) (ABIN361485)

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GRIN2B Antibody

Binding Specificity: pTyr1472

Specificity: Specific for endogenous levels of the ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Tyr1472. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: bovine, canine, chicken, human, non-human primate, zebra fish

Characteristics: Our NMDA NR2B Subunit (Tyr1472) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects NMDA NR2B Subunit (Tyr1472) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: ICC: 1:100

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-GRIN2B antibody (ABIN361485)

Knox, Brennan-Minnella, Lu, Yang, Nakazawa, Yamamoto, Swanson, Ferriero, Jiang: "NR2B phosphorylation at tyrosine 1472 contributes to brain injury in a rodent model of neonatal hypoxia-ischemia." in: Stroke; a journal of cerebral circulation, Vol. 45, Issue 10, pp. 3040-7, (2014) (PubMed).

Gladding, Sepers, Xu, Zhang, Milnerwood, Lombroso, Raymond: "Calpain and STriatal-Enriched protein tyrosine phosphatase (STEP) activation contribute to extrasynaptic NMDA receptor localization in a Huntington's disease mouse model." in: Human molecular genetics, Vol. 21, Issue 17, pp. 3739-52, (2012) (PubMed).

Castillo, Norcini, Baquero-Buitrago, Levacic, Medina, Montoya-Gacharna, Blanck, Dubois, Recio-Pinto: "The NMDA-evoked cytoplasmic calcium increase in adult rat DRG neuronal somata was potentiated by SP pretreatment in a PKC-dependent manner." in: Neuroscience, (2011) (PubMed).

Carreño, Walch, Dutta, Nedungadi, Cunningham: "Brain-Derived Neurotrophic Factor-Tyrosine Kinase B Pathway Mediates NMDA Receptor NR2B Subunit Phosphorylation in the Supraoptic Nuclei Following Progressive Dehydration." in: Journal of neuroendocrinology, Vol. 23, Issue 10, pp. 894-905, (2011) (PubMed).

Venkitaramani, Moura, Picciotto, Lombroso: "Striatal-enriched protein tyrosine phosphatase (STEP) knockout mice have enhanced hippocampal memory." in: The European journal of neuroscience, Vol. 33, Issue 12, pp. 2288-98, (2011) (PubMed).

Hicklin, Wu, Radcliffe, Freund, Goebel-Goody, Correa, Proctor, Lombroso, Browning: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, (2011) (PubMed).

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: GRIN2B

Background:

Synonyms: EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody

Description Logic: Our NMDA NR2B Subunit (Tyr1472) rabbit polyclonal phosphospecific primary antibody is produced . It detects NMDA NR2B Subunit (Tyr1472) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: GRIN2B

Molecular Weight: 180 kDa

Gene ID: 24410

UniProt: Q00960

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity