SYN1 antibody (pSer9)

Catalog No. ABIN361495

Product Details anti-SYN1 Antibody

Target: SYN1 (Synapsin I (SYN1))

Binding Specificity: pSer9

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for ~78k synapsin I doublet protein phosphorylated at Ser9. The antibody also weakly labels the ~55k synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment withphosphatase.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Predicted Reactivity: bovine, human, Xenopus, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-SYN1 antibody (ABIN361495)

Shulman, Stavsky, Fedorova, Mikulincer, Atias, Radinsky, Kahn, Slutsky, Gitler: "ATP binding to synaspsin IIa regulates usage and clustering of vesicles in terminals of hippocampal neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 3, pp. 985-98, (2015) (PubMed).

Shimomura, Nagamine, Hatano, Sueyoshi, Kameshita: "Identification of an endogenous substrate of zebrafish doublecortin-like protein kinase using a highly active truncation mutant." in: Journal of biochemistry, Vol. 147, Issue 5, pp. 711-22, (2010) (PubMed).

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: SYN1 (SYN1 Products)

Synonyms: SYN1a antibody, SYN1b antibody, SYNI antibody, Syn-1 antibody, SYN I antibody, si:dkey-90n12.3 antibody, synapsin I antibody, synapsin I L homeolog antibody, SYN1 antibody, Syn1 antibody, syn1.L antibody, syn1 antibody

Molecular Weight: '78 kDa

Gene ID: 281510

UniProt: P17599