PLK1 antibody (pSer482, pSer486, pSer490)

Catalog No. ABIN361516

This Rabbit Polyclonal antibody specifically detects PLK1 in WB. It exhibits reactivity toward Xenopus laevis.

Quick Overview for PLK1 antibody (pSer482, pSer486, pSer490) (ABIN361516)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PLK1 Antibody

Binding Specificity: pSer482, pSer486, pSer490

Specificity: Specific for the ~120k PLKK protein phosphorylated at Ser482,486,490.

Cross-Reactivity: Xenopus laevis

Predicted Reactivity: rat, mouse human, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Molecular Weight: '120 kDa

Gene ID: 380481

UniProt: P70032

Pathways: Cell Division Cycle, M Phase