PLK1 antibody (pSer482, pSer486, pSer490)

Catalog No. ABIN361516

This Rabbit Polyclonal antibody specifically detects PLK1 in WB and ELISA. It exhibits reactivity toward Xenopus laevis.

Quick Overview for PLK1 antibody (pSer482, pSer486, pSer490) (ABIN361516)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PLK1 Antibody

Binding Specificity: pSer482, pSer486, pSer490

Specificity: Specific for endogenous levels of the ~120k PLKK protein phosphorylated at Ser482,486,490.

Cross-Reactivity: Rat, Xenopus laevis

Predicted Reactivity: rat, mouse human, zebra fish

Characteristics: Our Polo-Like Kinase Kinase (Ser482,486,490) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Polo-Like Kinase Kinase (Ser482,486,490) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 of Xenopus polo-like kinase kinase (PLKK), conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background:

Synonyms: Polo like kinase kinase 1 antibody, Serine/threonine kinase 10 antibody, stk10 a antibody, xPlkk1 antibody

Description Logic: Our Polo-Like Kinase Kinase (Ser482,486,490) rabbit polyclonal phosphospecific primary antibody is produced . It detects Polo-Like Kinase Kinase (Ser482,486,490) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: PLKK1

Molecular Weight: 120 kDa

Gene ID: 380481

UniProt: B7ZR30

Pathways: Cell Division Cycle, M Phase