The antibody detects endogenous level of p95/NBS1 only when phosphorylated at serine 343.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer343 (S-L-S (p) -Q-G) derived from Human p95, NBS1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
KO Validated
NBN
Reactivity: Human
WB, IF, ICC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western blotting: 1:500-1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Drissi, Wu, Hu, Bockhold, Dome: "Telomere shortening alters the kinetics of the DNA damage response after ionizing radiation in human cells." in: Cancer prevention research (Philadelphia, Pa.), Vol. 4, Issue 12, pp. 1973-81, (2011) (PubMed).
Carrillo, Schneider, Taylor, Srinivasan, Kapoor, Bhatia: "Prominent oromandibular dystonia and pharyngeal telangiectasia in atypical ataxia telangiectasia." in: Cerebellum (London, England), Vol. 8, Issue 1, pp. 22-7, (2009) (PubMed).
Mutations in p95/NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.