Progesterone Receptor antibody (pSer190)

Catalog No. ABIN362013

The Rabbit Polyclonal anti-Progesterone Receptor antibody (ABIN362013) specifically detects Progesterone Receptor in WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for Progesterone Receptor antibody (pSer190) (ABIN362013)

Target: Progesterone Receptor (PGR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Progesterone Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Progesterone Receptor Antibody

Binding Specificity: pSer190

Specificity: The antibody detects endogenous level of Progesterone Receptor only when phosphorylated at serine 190.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer190 (G-L-S (p) -P-A) derived from Human Progesterone Receptor. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for Progesterone Receptor

Target: Progesterone Receptor (PGR)

Alternative Name: Progesterone Receptor

Background: Progesterone receptors (PRs) are nuclear hormone receptors of the NR3C class, which also includes mineralocorticoid,glucocorticoid and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins, which are shed upon activation. The major signaling pathway used by progesterone receptors is via direct DNA binding and transcriptional regulation of target genes. They can also signal by binding to other proteins, mainly with transcription factors such as NF-kappaB, AP-1 or STAT. Progesterone receptors are found in the female reproductive tract, mammary glands, brain and pituitary gland and receptor expression is induced by estrogen. Well established functions of progesterone receptors include ovulation, implantation, mammary gland development and maintenance of pregnancy. In addition,progesterone, signaling through the progesterone receptor, increases the ventilatory response of the respiratory centers to carbon dioxide and decreases arterial and alveolar PCO2 in the luteal phase of the menstrual cycle and during pregnancy. The human gene encoding the progesterone receptor has been localized to 11q22.

Molecular Weight: 99 kDa

NCBI Accession: NP_000917

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration