RPS6KA3 antibody (pThr348)
Quick Overview for RPS6KA3 antibody (pThr348) (ABIN362068)
Target
See all RPS6KA3 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- pThr348
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Specificity
- The antibody detects endogenous level of p90RSK only when phosphorylated at threonine 348.
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Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Immunogen
- Peptide sequence around phosphorylation site of pThr348 (S-R-T (p) -P-R) derived from Human p90RSK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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Isotype
- IgG
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Application Notes
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))
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Alternative Name
- p90RSK
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Background
- Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
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Molecular Weight
- 90 kDa
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NCBI Accession
- NP_001006
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UniProt
- Q15418
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Pathways
- MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
Target
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