FOXO4 antibody (pSer197)

Catalog No. ABIN362126

This Rabbit Polyclonal antibody specifically detects FOXO4 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 2+ publications

Quick Overview for FOXO4 antibody (pSer197) (ABIN362126)

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-FOXO4 Antibody

Binding Specificity: pSer197

Specificity: The antibody detects endogenous level of AFX only when phosphorylated at serine 197.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer197 (A-A-S (p) -M-D) derived from Human AFX. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-FOXO4 antibody (ABIN362126)

Pierce, Podlutskaya, Halloran, Hussong, Lin, Burbank, Hart, Galvan: "Over-expression of heat shock factor 1 phenocopies the effect of chronic inhibition of TOR by rapamycin and is sufficient to ameliorate Alzheimer's-like deficits in mice modeling the disease." in: Journal of neurochemistry, Vol. 124, Issue 6, pp. 880-93, (2013) (PubMed).

Zhan, Wang, Li, Xu, Sun, Xu: "Activation of Akt/FoxO signaling pathway contributes to induction of neuroprotection against transient global cerebral ischemia by hypoxic pre-conditioning in adult rats." in: Journal of neurochemistry, Vol. 114, Issue 3, pp. 897-908, (2010) (PubMed).

Target Details for FOXO4

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Alternative Name: AFX

Background: Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.

Molecular Weight: 65 kDa

NCBI Accession: NP_001164

UniProt: P98177

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation