VAV1 antibody (pTyr174)

Catalog No. ABIN362132

This Rabbit Polyclonal antibody specifically detects VAV1 in WB. It exhibits reactivity toward Human, Rat and Mouse.It has been mentioned in 1 publication

Quick Overview for VAV1 antibody (pTyr174) (ABIN362132)

Target: VAV1 (Vav 1 Oncogene (VAV1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAV1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VAV1 Antibody

Binding Specificity: pTyr174

Specificity: The antibody detects endogenous level of Vav only when phosphorylated at tyrosine 174.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr174 (E-I-Y (p) -E-D) derived from Human Vav. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-VAV1 antibody (ABIN362132)

Sekine, Ikeda, Tsuji, Yamamoto, Muromoto, Nanbo, Oritani, Yoshimura, Matsuda: "Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 12, pp. 7966-74, (2009) (PubMed).

Target Details for VAV1

Target: VAV1 (Vav 1 Oncogene (VAV1))

Alternative Name: Vav

Background: Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation

Molecular Weight: 95 kDa

NCBI Accession: NP_005419

UniProt: P15498

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, BCR Signaling