Merlin antibody (pSer518)

Catalog No. ABIN362336

This Rabbit Polyclonal antibody specifically detects Merlin in WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Merlin antibody (pSer518) (ABIN362336)

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Merlin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Merlin Antibody

Binding Specificity: pSer518

Specificity: The antibody detects endogenous level of Merlin only when phosphorylated at serine 518.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer518 (R-L-S (p) -M-E) derived from Human Merlin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for Merlin

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Alternative Name: Merlin

Background: Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity.

Molecular Weight: 69 kDa

NCBI Accession: NP_000259

UniProt: P35240

Pathways: Cell-Cell Junction Organization