Cortactin antibody (pTyr466)

Catalog No. ABIN362346

The Rabbit Polyclonal anti-Cortactin antibody is suitable to detect Cortactin in samples from Human and Mouse. It has been validated for WB.

Quick Overview for Cortactin antibody (pTyr466) (ABIN362346)

Target: Cortactin (CTTN)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cortactin Antibody

Binding Specificity: pTyr466

Specificity: The antibody detects endogenous level of Cortactin only when phosphorylated at tyrosine 466.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr466 (P-V-Y (p) -E-T) derived from Human CORTACTIN. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: Cortactin

Background: Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

Molecular Weight: 85 kDa

NCBI Accession: NP_005222

UniProt: Q14247

Pathways: MAPK Signaling