Aurora A antibody (pThr288)

Catalog No. ABIN362454

The Rabbit Polyclonal anti-Aurora A antibody has been validated for IHC. It is suitable to detect Aurora A in samples from Human and Mouse.

Quick Overview for Aurora A antibody (pThr288) (ABIN362454)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-Aurora A Antibody

Binding Specificity: pThr288

Specificity: The antibody detects endogenous level of Aurora A only when phosphorylated at threonine 288.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr288 (R-T-T (p) -L-M) derived from Human Aurora A. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: Aurora A

Background: Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity By similarity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.

Molecular Weight: 48 kDa

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization