Paxillin antibody (pTyr88)

Catalog No. ABIN362464

The Rabbit Polyclonal anti-Paxillin antibody (ABIN362464) specifically detects Paxillin in IF. The antibody is reactive with Human samples.

Quick Overview for Paxillin antibody (pTyr88) (ABIN362464)

Target: Paxillin (PXN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Immunofluorescence (IF)

Product Details anti-Paxillin Antibody

Binding Specificity: pTyr88

Specificity: The antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 88.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr88 (P-V-Y (p) -G-S) derived from Human Paxillin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: Paxillin

Background: An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

Molecular Weight: 68 kDa

NCBI Accession: NP_001074

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling