Bcl-2 antibody (AA 54-58)

Catalog No. ABIN362594

This Rabbit Polyclonal antibody specifically detects Bcl-2 in WB, IHC and IF. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for Bcl-2 antibody (AA 54-58) (ABIN362594)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 54-58

Specificity: The antibody detects endogenous level of total BCL-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 54-58 (G-H-T-P-H) derived from Human BCL-2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-Bcl-2 antibody (ABIN362594)

Pang, Xiong, Zhang, Wei, Chen, Yang: "Lentivirus-mediated Sirt1 shRNA and resveratrol independently induce porcine preadipocyte apoptosis by canonical apoptotic pathway." in: Molecular biology reports, Vol. 40, Issue 1, pp. 129-39, (2012) (PubMed).

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: BCL-2

Background: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Molecular Weight: 26 kDa

NCBI Accession: NP_000624

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling