Phospholipase C gamma 1 antibody (AA 781-785)

Catalog No. ABIN362720

This Rabbit Polyclonal antibody specifically detects Phospholipase C gamma 1 in WB. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for Phospholipase C gamma 1 antibody (AA 781-785) (ABIN362720)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Phospholipase C gamma 1 Antibody

Binding Specificity: AA 781-785

Specificity: The antibody detects endogenous level of total PLCg1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 781-785 (G-F-Y-V-E) derived from Human PLCg1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-Phospholipase C gamma 1 antibody (ABIN362720)

Pan, Dong, Wang, Yang, Zhang, Li, Zhang, Yang, Li, Tan, Wang: "Signal regulatory protein ? negatively regulates mast-cell activation following Fc?RI aggregation." in: European journal of immunology, Vol. 43, Issue 6, pp. 1598-607, (2013) (PubMed).

Hsieh, Liu, Hsu, Jayakumar, Chou, Wang, Hsiao, Sheu et al.: "Inhibition of vascular smooth muscle cell proliferation by the vitamin E derivative pentamethylhydroxychromane in an in vitro and in vivo study: pivotal role of hydroxyl radical-mediated PLCgamma1 ..." in: Free radical biology & medicine, Vol. 49, Issue 5, pp. 881-93, (2010) (PubMed).

Target Details for Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Alternative Name: PLCgamma1

Background: PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.

Molecular Weight: 155 kDa

NCBI Accession: NP_002651

UniProt: P19174

Pathways: RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling