Paxillin antibody (PXN) (AA 86-90)

Details for Product anti-PXN Antibody No. ABIN363106
Antigen
  • AW108311
  • AW123232
  • Pax
  • CG18061
  • CG18576
  • CG31794
  • CT40481
  • CT42454
  • DPaxillin
  • DPxn
  • DPxn37
  • Dmel\\CG31794
  • Dpax
  • DpaxA
  • PDLP
  • dPax
  • pax
  • wu:fw71f12
  • PXN
  • LOC100220858
  • paxillin
  • Paxillin
  • paxillin S homeolog
  • paxillin a
  • PXN
  • Pxn
  • Pax
  • pxn.S
  • pxna
  • LOC696336
  • pxn
Alternatives
anti-Human Paxillin antibody for Western Blotting
Epitope
AA 86-90
67
65
38
30
24
16
15
14
11
10
7
6
6
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
453
245
230
27
12
10
8
5
5
5
4
3
1
1
1
1
Host
Rabbit
423
40
2
Clonality
Polyclonal
Conjugate
This Paxillin antibody is un-conjugated
15
13
13
13
13
13
13
6
6
4
4
4
4
4
2
2
2
2
2
1
Application
Immunofluorescence (IF), Western Blotting (WB)
335
127
113
53
47
45
27
19
10
8
4
2
1
Options
Immunogen Peptide sequence around AA 86-90 (P-V-Y-G-S) derived from Human Paxillin. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total Paxillin protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen .
Plasmids, Primers & others Plasmids, Primers & others Paxillin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Paxillin (PXN Antibody Abstract)
Background Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Molecular Weight 68 kDa
NCBI Accession NP_001074
UniProt P49023
Pathways MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Wang, Bi, Ampah, Zhang, Li, Jiao, Wang, Ba, Zeng: "Lipid raft regulates the initial spreading of melanoma A375 cells by modulating ?1 integrin clustering." in: The international journal of biochemistry & cell biology, Vol. 45, Issue 8, pp. 1679-89, 2013 (PubMed).

Background publications Turner: "Paxillin interactions." in: Journal of cell science, Vol. 113 Pt 23, pp. 4139-40, 2001 (PubMed).

Bellis, Miller, Turner: "Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17437-41, 1995 (PubMed).

Schaller, Parsons: "pp125FAK-dependent tyrosine phosphorylation of paxillin creates a high-affinity binding site for Crk." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2635-45, 1995 (PubMed).

Burridge, Turner, Romer: "Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly." in: The Journal of cell biology, Vol. 119, Issue 4, pp. 893-903, 1992 (PubMed).

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