GFP antibody (HRP)

Catalog No. ABIN364147

This Mouse Monoclonal antibody specifically detects GFP in WB, ELISA and IHC. It exhibits reactivity toward Aequorea victoria.

Quick Overview for GFP antibody (HRP) (ABIN364147)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 1003

Product Details anti-GFP Antibody (HRP)

Specificity: Recognizes wild type and recombinant Green Fluorescent Protein. Does not recognize enhanced forms of GFP. Expected to detect GFP containing inserts by Immunofluorescence Microscopy on prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-peroxidase, and purified and partially purified GFP (Aequorea victoria) serum.

Purification: Protein A purified

Immunogen: GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IHC (1:500 - 1:2500), WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA (sandwich or capture): 1:10000-1:50000. Western Blot: 1:1000-1:5000. Immunohistochemistry: 1:500-1:2500. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Aequorea victoria

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Thimerosal, 40 % glycerol.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light.

Storage: 4 °C,-80 °C

Storage Comment: Short term: 4°C
Long term: -70°C
Protect from light.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212