Collagen IV antibody

Catalog No. ABIN370831

This Mouse Monoclonal antibody specifically detects Collagen IV in IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for Collagen IV antibody (ABIN370831)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 2F11

Product Details anti-Collagen IV Antibody

Specificity: This antibody reacts with Human Type IV Collagen.

Characteristics: Synonyms: COL4A1

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/2,000-1/8,000. Immunohistochemistry: < / = 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV

Background: Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.Synonyms: COL4A1

Gene ID: 1282

UniProt: P02462