GFP antibody (N-Term)

Catalog No. ABIN371877

This Mouse Monoclonal antibody specifically detects GFP in WB and EIA. It exhibits reactivity toward Aequorea victoria.

Quick Overview for GFP antibody (N-Term) (ABIN371877)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: AT2G5

Product Details anti-GFP Antibody

Binding Specificity: AA 1-238, N-Term

Characteristics: Synonyms: Green fluorescent protein, GFP-Tag

Purification: Protein-G affinity chromatography

Immunogen: Recombinant GFP (1-238 aa) purified from E. coli

Isotype: IgG2a

Application Details

Application Notes: ELISA. Western blot (1: 1,000 - 1: 2,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Synonyms: GFP-Tag, Green fluorescent protein

UniProt: P42212